Bacteria and Resistance to Antibiotics. Identification using Nanotechnology and Microfluidics
Key words: microfluidic diagnostic, bacteria, antibiotic resistance.
- Companies: Coris BioConcept (Coordinator), WOW Company
- Research centers: ULB (Hôpital Erasme, Laboratoire de Microbiologie/Département de Biologie Clinique), UCL-Mont Godinne (Laboratoire de Microbiologie)
Topic of the project
The ultimate goal is to develop a test to identify within 1 h the main kinds of pathogenic bacteria and resistance parameters to antibiotics most commonly encountered in cases of bacteremia in order to allow doctors to quickly define the most adequate therapy, based on technical data rather than on empiricism.
Blood cultures will be the samples of choice. The technology involves the extraction of DNA from pathogenic bacteria followed by multiplex hybridization on a microfluidic chip of extracted DNA. Detection will be carried out with nanoparticles such as gold colloids, latex microspheres or fluorescent carbon nanotubes or fluorescent molecules.
The purpose of the research is to develop a first generation of this platform to demonstrate the feasibility with a limited but clinically relevant targets.
Research will therefore focus on three bacterial species and three resistance mechanisms of representative isolates that are frequent and of clinical and therapeutic importance in sepsis and recognized to be difficult to treat, i.e. Staphylococcus aureus and methicillin resistance (mecA), Escherichia coli and resistance induced by β-lactamases (extended spectrum CTX-M) and Pseudomonas aeruginosa with resistance to imipenem by metallo-β-lactamases [IMP and VIM].
This is a 4-year project with a total budget of M€ 2.8.
Thierry Leclipteux (Coordinator of the project)